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目前,微型高效液相色谱法很引人注意。微型住(内径0.5～2 mm)具有较高的色谱分离效能,而且可以大大地减少流动相的消耗。由于使用了小内径的微型柱,高效液相色谱仪需要作特殊的更改,进样器和捡测池的体积必须减少,泵必须精确地输送如10μl/min 这样低的流量。本文报道用微型柱(50cm×1mm 内径)和经更改的 Waters 高效液相色谱系统,分析32种自由型和结合型胆汁酸,不饱和甾醇和类固醇酸的情况。Waters6000A 型泵连有 Waters 电子流分离器可输送10～90μl/min 的流量,WatersU 6 K 型进样器配有10μl 进样环,该环连在不锈钢微型柱(HR SM-50-C18,C-M
At present, miniature high performance liquid chromatography is very attractive. Micro-living (0.5 ~ 2 mm in inner diameter) has high performance of chromatographic separation, and can greatly reduce the consumption of mobile phase. Because of the small inner diameter of the miniature column, the HPLC needs special changes, the volume of the sampler and cell must be reduced, and the pump must deliver precisely as low a flow as 10 μl / min. This paper reports the analysis of 32 free and bound bile acids, unsaturated sterols, and steroid acids using a microcolumn (50 cm x 1 mm id) and a modified Waters High Performance Liquid Chromatography system. The Waters 6000 Series pump was connected to a Waters flow separator delivering 10 to 90 μl / min. The Waters U 6 K was equipped with a 10 μl injection loop attached to a stainless steel mini column (HR SM-50-C18, C-M