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目的 探讨α -干扰素 (IFN -α)治疗慢性乙型肝炎的细胞免疫机制。方法 收集我院慢性乙型肝炎患者 3 0例 ,分别在IFN -α治疗前 ,治疗后 1个月、 3个月、 6个月和 9个月留取血标本 ,用放免法 (RIA)检测血清肿瘤坏死因子 (TNF)、白细胞介素 - 6(IL - 6)水平 ,同时采用双抗体夹心酶联免疫吸附试验(ELISA)检测血清白细胞介素 - 4 (IL - 4 )水平 ,并与 3 0例正常献血员的各值分别进行对照。结果 与正常对照组相比 ,慢性乙型肝炎患者血清TNF、IL - 6水平升高 (P <0 0 1) ,IL - 4水平降低 (P <0 0 1) ;经IFN -α治疗后 ,血清TNF水平较治疗前有所下降 ,但仍高于对照组 (P <0 0 5 ) ,而血清IL - 4水平明显升高 (P <0 0 1)。结论 IFN -α通过诱导体内细胞因子变化 ,参与细胞免疫调控
Objective To investigate the cellular immune mechanism of interferon α (IFN-α) in the treatment of chronic hepatitis B. Methods Thirty patients with chronic hepatitis B in our hospital were enrolled. Blood samples were collected before treatment, 1 month, 3 months, 6 months and 9 months after IFN-α treatment, respectively. RIA Serum levels of tumor necrosis factor (TNF) and interleukin - 6 (IL - 6) were detected by enzyme - linked immunosorbent assay (ELISA). The level of IL - The values of 0 normal blood donors were compared respectively. Results Compared with the normal control group, the levels of serum TNF and IL - 6 in patients with chronic hepatitis B increased (P <0.01) and the levels of IL - 4 decreased (P <0.01). After treatment with IFN-α, Serum TNF levels were lower than those before treatment, but still higher than those of the control group (P <0.05), while serum IL - 4 levels were significantly increased (P <0.01). Conclusion IFN-α is involved in cellular immune regulation by inducing cytokine changes in vivo