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目的:探讨PTEN、p53及hTERT在Ⅰ型子宫内膜癌中的表达,为子宫内膜癌的早期诊断和治疗提供理论依据。方法:应用免疫组织化学SABC法同步检测49例子宫内膜癌中的p53、PTEN、hTERT的表达,取同期49例正常的子宫内膜组织做对照,数据采用SPSS13.0软件处理,计数资料进行X2检验,等级资料进行秩和检验。结果:PTEN蛋白在子宫内膜癌中阳性表达率为40.8%,低于正常子宫内膜组97.9%,且阳性表达与子宫内膜癌的组织分级、FIGO分期、淋巴转移、肌层浸润深度呈负相关;相反,hTERT和p53两种蛋白在子宫内膜癌组阳性表达率(67.3%、38.8%)均高于正常子宫内膜组,并子宫内膜癌恶性程度及FIGO分期呈正相关。结论:与正常子宫内膜相比,PTEN在子宫内膜癌中表达减少,而P53及hTERT呈表达升高,三者均可作为子宫内膜诊断及判定预后的标记物。
Objective: To investigate the expression of PTEN, p53 and hTERT in type Ⅰ endometrial carcinoma and to provide a theoretical basis for the early diagnosis and treatment of endometrial carcinoma. Methods: The expression of p53, PTEN and hTERT in 49 cases of endometrial carcinoma was detected synchronously by immunohistochemical SABC method. 49 cases of normal endometrium were taken as control. The data were processed by SPSS13.0 software and counting data X2 test, grade data rank sum test. Results: The positive expression rate of PTEN protein in endometrial carcinoma was 40.8%, which was lower than that in normal endometrium group (97.9%). The positive expression of PTEN protein was correlated with the histological grade of the endometrial carcinoma, FIGO staging, lymphatic metastasis, depth of myometrial invasion In contrast, the positive rates of hTERT and p53 in endometrial carcinoma (67.3%, 38.8%) were significantly higher than those in normal endometrium, and were positively correlated with the malignancy of the endometrial carcinoma and the FIGO stage. Conclusions: Compared with normal endometrium, the expression of PTEN decreased in endometrial carcinoma, while the expression of P53 and hTERT increased, all of which could be used as the marker of endometrial diagnosis and prognosis.