[目的]观察鼓膜穿孔对噪声性听损伤的豚鼠脑干诱发电位和畸变产物耳声发射的影响。[方法]10只豚鼠分别行右鼓膜穿孔,穿孔面积约占整个鼓膜的25%,左耳为对照耳。每天定时暴露于稳态噪声4h,连续暴露6d,观察左右两耳听觉脑干诱发电位(auditory brainstem response,ABR)和畸变产物耳声发射(distort product otoacoustic e-mission,DPOAE)的变化。[结果]两耳均出现ABR反应阈的上升和DPOAE幅值下降,脱离噪声暴露一段时间后,正常耳可恢复,穿孔耳仍有听阈的暂时性阈移,二者差异有统计学意义。[结论]鼓膜穿孔后,耳蜗外毛细胞对噪声更敏感,听阈的暂时性阈移恢复延迟。 [Objective] To observe the effects of tympanic membrane perforation on brainstem response and distortion product otoacoustic emissions in guinea pigs with noise-induced hearing impairment. [Method] Ten guinea pigs were perforation of the right tympanic membrane, the perforation area accounted for about 25% of the entire tympanic membrane, the left ear was the control ear. The daily exposure to steady-state noise was performed for 4 hours and continuous exposure for 6 days. The changes of auditory brainstem response (ABR) and distortion product otoacoustic e-mission (DPOAE) were observed. [Results] The ABR response threshold and DPOAE amplitude decreased in both ears, and the normal ears could recover after leaving the noise exposure for a period of time. There was still a temporary thresholding of hearing threshold in the ears, the difference was statistically significant. [Conclusion] After perforation of the tympanic membrane, the outer hair cells of the cochlea are more sensitive to noise and the transient threshold shift of the hearing threshold is delayed.