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目的:建立银翘伤风胶囊中HPLC法测定牛蒡苷、连翘苷的含量。方法:Agilent-ODS C_(18)柱(250mm×4.6mm,5μm),流动相为甲醇-水(28:72);检测波长278nm;流速1.0ml·min~(-1),柱温35℃。结果:牛蒡苷在0.3～3.0μg范围内线性关系良好(r=0.999 8),平均回收率:100.3%,RSD2.0%(n=5),连翘苷在0.02～0.15μg范围内线性关系良好(r=0.9998),平均加样率:100.0%,RSD2.0%(n=5)。结论:结果准确,便于操作,可作为银翘伤风胶囊的质量控制方法。
Objective: To establish the HPLC method for the determination of aricin and forsythin in Yinqiao Shifeng Capsules. Method: Agilent-ODS C 18 column (250mm×4.6mm, 5μm), mobile phase methanol-water (28:72); detection wavelength at 278nm; flow rate 1.0ml·min~(-1), column temperature at 35°C . RESULTS: The linear relationship was good in the range of 0.3-3.0 μg (r=0.999 8), the average recovery rate was 100.3%, the RSD was 2.0% (n=5), and the linear relationship of forsythin was in the range of 0.02-0.15 μg. Good (r=0.9998), average addition rate: 100.0%, RSD 2.0% (n=5). Conclusion: The result is accurate, easy to operate, and can be used as a quality control method for Yinqiao Shifeng Capsule.