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目的优化肠道病毒71型(Enterovirus 71,EV71)在Vero细胞中的培养条件。方法分别采用吸附法和直接接种法将EV71接种于Vero细胞中进行增殖,检测病毒滴度;选择最佳接种方法接种病毒,考察血清浓度、收毒方式、病毒接种量(MOI)和收毒时间对病毒滴度的影响。结果将MOI为0.02～0.2的病毒直接接种于不含血清的培养基中,培养60 h,胞外收毒,可获得较高的病毒滴度。结论优化了EV71在Vero细胞中的培养条件,为EV71的大规模培养及其疫苗研发奠定了基础。
Objective To optimize the culture conditions of Enterovirus 71 (EV71) in Vero cells. Methods The EV71 cells were inoculated into Vero cells by the method of adsorption and direct inoculation respectively to proliferate and detect the virus titer. The optimal inoculation method was selected to inoculate the virus, and the serum concentration, drug collection mode, virus inoculum size (MOI) Impact on virus titer. Results The virus with the MOI of 0.02-0.2 was directly inoculated into the serum-free medium. After culturing for 60 h, the virus was absorbed extracellularly to obtain a higher virus titer. Conclusion The culture conditions of EV71 in Vero cells were optimized, which laid the foundation for the large-scale culture of EV71 and its vaccine development.