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目的探讨维吾尔族卡波西肉瘤(Kaposi’s sarcoma,KS)患者皮损组织内KSHV-vFLIP基因表达水平与临床特征的相关性。方法选取29例维吾尔族卡波西肉瘤患者和12例血管瘤(hemangioma)患者、11例健康对照患者皮损石蜡包埋组织,反转录-聚合酶链反应(RT-PCR)分析KSHV-vFLIP基因mRNA表达水平。结果 KS患者KSHV-vFLIP基因表达水平(4.436±0.508)显著高于健康对照组(1.145±0.112,P<0.05),其中斑块型组KSHV-vFLIP基因表达水平(8.104±2.225)显著高于结节型组(3.971±0.371,P<0.05)和斑片组(2.774±0.867,P<0.05)。而KS组(4.436±0.507)与血管瘤组(6.343±0.949)差异无统计学意义(P>0.05),且HIV携带组(3.739±0.816)与非HIV携带组(4.618±0.604)差异无统计学意义(P>0.05)。结论在维吾尔族卡波西肉瘤患者组织中,KSHV-vFLIP基因表达水平与瘤体体积正相关。
Objective To investigate the relationship between KSHV-vFLIP gene expression and clinical features in the lesions of Kaposi’s sarcoma (KS). Methods Totally 29 Uighur Kaposi’s sarcoma patients and 12 hemangioma patients were enrolled in this study. Paraffin-embedded paraffin embedded tissues of 11 healthy controls were collected. Reverse transcription-polymerase chain reaction (RT-PCR) analysis of KSHV-vFLIP Gene mRNA expression level. Results The KSHV-vFLIP gene expression level in KS patients (4.436 ± 0.508) was significantly higher than that in healthy controls (1.145 ± 0.112, P <0.05). The KSHV-vFLIP gene expression level in KS group (8.104 ± 2.225) (3.971 ± 0.371, P <0.05) and the patch group (2.774 ± 0.867, P <0.05). There was no significant difference between KS group (4.436 ± 0.507) and hemangioma group (6.343 ± 0.949) (P> 0.05), and there was no statistical difference between HIV-carrying group (3.739 ± 0.816) and non-HIV group Significance (P> 0.05). Conclusion KSHV-vFLIP gene expression is positively correlated with tumor volume in Uighur Kaposi’s sarcoma.