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本文从亮叶杨桐中分离提取出黄酮类生物活性物质,对进行小鼠体内抗肿瘤试验和小鼠S-180肿瘤细胞中p53基因表达抑制实验。研究结果表明,亮叶杨桐中总黄酮含量达28.4％,黄酮提取物在剂量50mg·kg~(-1)时对小鼠肉瘤S-180的抑瘤率为64.0％,对艾氏腹水癌(EAC)Ⅰ小鼠的生命延长率为51.2％,有显著的抗肿瘤作用,并且药效与用药量有关。同时发现,黄酮类提取物的抗肿瘤活性优于化疗药物呋喃氟尿嘧啶(Ftorafur)。RT-PCR实验结果表明,在剂量500mg·kg~(-1)时,黄酮类提取物可以明显抑制小鼠S-180肿瘤细胞中突变型p53基因的转录活性,其抑制程度和呋喃氟尿嘧啶相同。
In this paper, we isolated and extracted flavonoid bioactive substances from the leaves of Populus euphratica, and performed anti-tumor experiments in mice and inhibition experiments of p53 gene expression in mouse S-180 tumor cells. The results showed that the content of total flavonoids in Populus davidiana leaves reached 28.4%, and the inhibitory rate of flavonoid extract against mouse sarcoma S-180 at a dose of 50 mg·kg -1 was 64.0%. Escherichia coli cancer (EAC) The life extension rate of )I mice is 51.2%, which has a significant anti-tumor effect, and the efficacy is related to the amount of medication. At the same time, it was found that the antitumor activity of flavonoid extracts was superior to that of the chemotherapy drug Ftorafur. The results of RT-PCR showed that the flavonoid extract can significantly inhibit the transcriptional activity of mutant p53 gene in mouse S-180 tumor cells at the dose of 500 mg·kg -1 , and the inhibition degree is the same as that of furan fluorouracil.