目的　了解在实验室条件下钩端螺旋体的生长规律 ,为进一步开展蛋白质组和基因表达谱的研究做准备。方法　使用pH为 7 4的EMJH液体培养基 ,在 30℃条件下置摇床上以 90r/min对该菌进行通气振荡培养 ,记录不同时间点的活菌数和培养物的光密度。结果　研究发现 ,钩体的生长与其它细菌培养相似 ,也有静止期、对数生长期、稳定期和衰老期。在该培养条件下 ,钩端螺旋体的对数生长期为培养的第 12～ 5 4h。在对数生长期 ,钩端螺旋体复制一代所需的时间约为 11 35h。并且 ,钩端螺旋体活菌计数和光密度值OD60 0 之间有较好的相关性。结论　本文比较详细地描述了钩端螺旋体的生长参数—活菌数和OD60 0 ,并找到了钩端螺旋体生长的对数生长期 ,在严格控制试验条件的情况下 ,可以使用测定液体培养物的OD60 0值的方法来推算菌体数 ,代替菌体计数的方法 Objective To understand the growth of Leptospira in laboratory conditions and to prepare for the further study of proteome and gene expression profile. Methods EMJH liquid medium with pH value of 74 was used to shake-culture the bacteria at 90r / min on a rocking bed at 30 ℃. The viable count and the optical density of the culture were recorded at different time points. Results The study found that the growth of leptospira was similar to that of other bacterial cultures, and there were quiescent, logarithmic, stable and senescent phases. Under this culture condition, the logarithmic growth phase of Leptospira was the 12th to 54th hour of culture. In logarithmic growth phase, the time required for Leptospira to reproduce a generation is about 1135 hours. Also, there was a good correlation between viable count of Leptospira and OD60 0. Conclusions In this paper, the growth parameters of Leptospira-viable cells and OD60 0 were described in more detail and the logarithmic growth phase of Leptospira was found. Under strict control of the test conditions, OD60 0 value method to calculate the number of bacteria, instead of bacterial count method