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本文介绍一种制备高效价链球菌溶血素“O”的方法,具体作法如下: 一、培养基: 1.基础液:将20g(月示)蛋白胨、5 g氯化钠溶于1L牛肉消化液中,校正pH至7.8,分转烧瓶,每大瓶,500ml,每小瓶50m1,高压蒸汽15磅15分钟灭茵。 2.10g dl葡萄糖液:将2g葡萄糖溶于20ml蒸馏水中,高压蒸汽10磅15分钟灭茵 3.20g dl碳酸氢钠液:将碳酸氢钠10g溶于50ml蒸馏水中,高压蒸汽15磅15分钟灭菌。 1.临用前于500ml基础液中加入10g dl葡萄糖液
This article describes a method for the preparation of high-price streptolysin “O” method, as follows: First, the medium: 1. Basal fluid: 20g (monthly) peptone, 5g sodium chloride dissolved in 1L beef digest The pH was adjusted to 7.8 and the flask was divided into separate flasks, 500 ml for each vial, 50 ml for each vial and 15 pounds for 15 minutes for autoclaving. 2.10g dl glucose solution: Dissolve 2g of glucose in 20ml distilled water, 10lbs of high-pressure steam for 15mins to extinguish 3.20g dl sodium bicarbonate solution: Dissolve 10g sodium bicarbonate in 50ml distilled water and autoclave 15lbs for 15 minutes . 1. Before use in 500ml base solution by adding 10g dl glucose solution