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目的观察银杏叶提取物(GBE)对小鼠耐缺氧能力的影响。方法用乙醇加热回流银杏叶,提取总黄酮,以芦丁标准品为对照,络合紫外分光光度法测总黄酮的含量。小鼠随机分为五个组,连续灌胃7 d后,做耐缺氧实验,记录小鼠每5 m in的耗氧量、总耗氧量和存活时间。结果不同剂量的GBE均能增加小鼠在密闭缺氧条件下的总耗氧量和存活时间,且呈正相关性,高剂量组与阴性对照组比较差异有统计学意义(P<0.05)。结论 GBE可提高小鼠的耐缺氧能力,减轻缺氧对机体的损伤。
Objective To observe the effect of ginkgo biloba extract (GBE) on hypoxia tolerance in mice. Methods The ginkgo biloba was refluxed with ethanol and the total flavonoids were extracted. The standard of rutin was used as control, and the content of total flavonoids was determined by UV spectrophotometry. Mice were randomly divided into five groups. After continuous gavage for 7 days, the mice were subjected to hypoxia tolerance test. The oxygen consumption, total oxygen consumption and survival time of mice were recorded every 5 mins. Results Different dosages of GBE increased the total oxygen consumption and survival time of mice under the condition of closed hypoxia, and there was a positive correlation between them. The difference between the high dose group and the negative control group was statistically significant (P <0.05). Conclusion GBE can improve hypoxia tolerance in mice and reduce the damage to the body caused by hypoxia.