作者研制了一种人二倍体细胞无血清培养液(RITC78-6),含有四种生长因子,即小鼠上皮生长因子、胰岛素、转铁蛋白和三碘甲状腺氨酸。本文探讨了人二倍体成纤维细胞无血清培养中生长因子和氢化考的松的作用。据报告,生长因子促进细胞繁殖的作用,必须有血清或牛血清蛋白的存在,虽然生长因子不能完全代替血清,但可减少对血清的需要量。另外,少数报告指出,无血清培养 The authors developed a human diploid serum-free culture medium (RITC78-6) containing four growth factors, namely mouse epithelial growth factor, insulin, transferrin and triiodothyronine. This article explored the role of growth factors and hydrocortisone in serum-free culture of human diploid fibroblasts. It has been reported that the growth factor promotes cell proliferation by the presence of serum or bovine serum albumin. Although growth factors do not completely replace serum, they reduce serum requirements. In addition, a few reports indicate that serum-free culture