论文部分内容阅读
为了探讨IL－11基因修饰成纤维细胞的体外促造血活性及其在造血系统基因治疗中的应用前景．方法：将含IL－11cDNA的逆转录病毒载体转染小鼠成纤维细胞系NIH－3T3细胞，获得了高表达IL－11的亚克隆1E7，通过造血祖细胞集落形成实验及细胞增殖实验对1E7细胞在体外的促造血活性做了初步的鉴定．结果：1E7培养上清或1E7细胞作为饲养细胞，在体外培养体系中均能明显地促进骨髓细胞GM－CFU，Meg－CFU和Mix－CFU的形成及骨髓细胞增殖．结论：IL－11cDNA基因转染的成纤维细胞能持续、稳定地表达造血因子目的基因，在体外具有促造血祖细胞集落形成及增殖的活性，是造血因子基因治疗中较为理想的靶细胞．
In order to investigate the in vitro hematopoietic activity of IL-11 gene modified fibroblasts and its potential application in hematopoietic system gene therapy. Methods: The mouse fibroblast cell line NIH-3T3 was transfected with the retrovirus vector containing IL-11 cDNA. The subclone 1E7 with high expression of IL-11 was obtained. The hematopoietic progenitor colony formation assay and cell proliferation assay were used to detect the expression of 1E7 Cells in vitro to promote hematopoietic activity made a preliminary identification. Results: 1E7 culture supernatant or 1E7 cells as feeder cells could obviously promote the formation of bone marrow cells GM-CFU, Meg-CFU and Mix-CFU and the proliferation of bone marrow cells in vitro. CONCLUSION: The fibroblasts transfected with IL-11 cDNA can consistently and stably express the target gene of hematopoietic cells and have the activity of promoting colony formation and proliferation of hematopoietic progenitor cells in vitro and is an ideal target cell for hematopoietic gene therapy.