论文部分内容阅读
目的:分析miR-30a-5p与miR-211在结直肠癌组织中的表达，探讨二者表达与结直肠癌临床病理特征及预后的关系。方法:回顾性分析2013年2月至2015年12月在山西省晋中市第二人民医院治疗的结直肠癌患者80例，术中收集结直肠癌组织及相应癌旁组织(距病灶≥3 cm)。采用实时荧光定量(qRT-PCR)法检测组织中miR-30a-5p与miR-211表达水平，分析二者表达与结直肠癌患者临床病理特征及预后的关系。结果:结直肠癌组织中miR-30a-5p、miR-211表达水平均显著低于癌旁组织(n P0.05)，与分化程度、肿瘤大小、浸润程度、TNM分期、淋巴结转移有关(n P<0.05)。在结直肠癌组织中miR-30a-5p与miR-211表达水平呈正相关(n r＝0.337，n P<0.05)。miR-30a-5p、miR-211低表达组3年存活率均显著低于miR-30a-5p、miR-211高表达组(n P<0.05)。低分化、高TNM分期、淋巴结转移、miR-30a-5p低表达、miR-211低表达是影响结直肠癌患者不良预后的独立危险因素(n P<0.05)。n 结论:miR-30a-5p、miR-211在结肠癌组织中均呈低表达，二者与肿瘤分化程度、淋巴结转移等临床病理特征及预后有关，可能共同作用，影响结直肠癌发生发展。“,”Objective:To analyze the expression of miR-30a-5p and miR-211 in colorectal cancer tissues, and to explore the relationships between the expression of miR-30a-5p, miR-211 and the clinicopathological characteristics, prognosis of colorectal cancer.Methods:80 patients with colorectal cancer treated in our hospital from February 2013 to December 2015 were retrospectively analyzed, and colorectal cancer tissues and corresponding adjacent tissues (≥3 cm far from the lesion) were collected during operation. Real-time fluorescence quantitative analysis (qRT-PCR) was used to detect the expression levels of miR-30a-5p and miR-211 in tissues, and the relationships between the expressions of miR-30a-5p, miR-211 and the clinicopathological characteristics, prognosis of colorectal cancer patients were analyzed.Results:The expression levels of miR-30a-5p and miR-122 in colorectal cancer tissues were significantly lower than those in adjacent tissues (n P0.05), but related to differentiation, size of tumors, degree of invasion, tumor node metastasis (TNM) stage and lymph node metastasis (n P<0.05). There was a positive correlation between the expressions of miR-30a-5p and miR-211 in colorectal cancer (n r=0.337, n P<0.05). The 3-year survival rates of the low-expression groups of miR-30a-5p and miR-211 were significantly lower than those of the high-expression groups of miR-30a-5p and miR-211 (alln P<0.05). Low differentiation, high TNM stage, lymph node metastasis, low expression of miR-30a-5p and low expression of miR-211 were independent risk factors for poor prognosis of colorectal cancer patients (n P<0.05).n Conclusions:The expressions of miR-30a-5p and miR-211 are low in colorectal cancer tissues, both of them are related to the clinicopathological characteristics and prognosis of colorectal cancer, such as the degree of differentiation of tumors, lymph node metastasis and so on, which may play a common role in influencing the occurrence and development of colorectal cancer.