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目的 了解猪在禽H9N2亚型流感病毒感染人中的作用。方法 用RT PCR扩增目的基因,用PGEM T Vector(美国Promega公司) 4℃过夜连接,重组质粒转入dH5α细菌,筛选阳性菌落,酶切鉴定,送六合通公司自动测序,然后进行进化树分析。结果 两株山东猪H9N2毒株基因组与人及禽分离出的H9N2病毒均有差异,中国内地从人分离出的H9N2毒株的基因组接近鸡的毒株,而香港特区从人分离出的接近鹌鹑的毒株;禽H9N2毒株不仅宿主范围广,同时其基因组具有多样性。结论 禽H9N2亚型毒株是直接感染人,而不是通过所谓的中间宿主猪,然后再感染人。
Objective To understand the role of pigs in the infection of H9N2 avian influenza virus in humans. Methods The target gene was amplified by RT-PCR and ligated overnight at 4 ℃ with PGEM T Vector (Promega, USA). The recombinant plasmid was transfected into dH5α bacteria. The positive colonies were screened and identified by enzyme digestion, and then sent to Liuhetong Company for automatic sequencing. Then phylogenetic tree analysis . Results The genome of H9N2 strain in Shandong pig was different from the H9N2 virus isolated from humans and poultry. The H9N2 strain isolated from humans in China had a genome closer to that of the chicken strain, whereas the isolated from Hong Kong SAR was quail The avian H9N2 strain not only has a wide range of hosts but also has a diversity of genomes. Conclusion Avian H9N2 subtype strains directly infect humans rather than passing through so-called intermediate host pigs and then infecting humans.