用荧光光谱法研究了人血清白蛋白与槲皮素的结合反应,发现槲皮素对人血清白蛋白的荧光有很强的猝灭作用,求得它同人血清白蛋白的离解常数(k_D=5.43×10~(-5)。鉴于人血清白蛋白与槲皮素之间可发生能量转移,利用荧光共振能量转移原理测定人血清白蛋白的色氨酸与槲皮素之间的距离,提出了复合物结合模型并探讨了其结合机理。 The binding reaction between human serum albumin and quercetin was studied by fluorescence spectroscopy. It was found that quercetin has a strong quenching effect on the fluorescence of human serum albumin, and the dissociation constant of human serum albumin was obtained (k_D= 5.43×10~(-5).In view of the energy transfer between human serum albumin and quercetin, the fluorescence resonance energy transfer principle was used to determine the distance between tryptophan and quercetin in human serum albumin. The composite binding model and its binding mechanism were explored.