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目的系统研究蒙古鸦葱的抗肿瘤活性成分。方法使用硅胶H、Sephadex LH-20、ODS和HPLC分离、纯化,根据理化性质和光谱分析鉴定化合物的结构。采用MTT法和SRB法进行活性跟踪和单体化合物的抗肿瘤活性筛选。结果分离鉴定了15个三萜类化合物,分别为羽扇豆醇(1)、白桦脂醇(2)、白桦脂酸(3)、乙酰羽扇豆醇(4)、23Z-3β-acetoxyeupha-7,23-diene-25-ol(5)、达玛二烯醇乙酸酯(6)、齐墩果烷乙酸酯(7)、蒲公英甾醇(8)、蒲公英甾醇乙酸酯(9)、伪蒲公英甾醇(10)、伪蒲公英甾醇乙酸酯(11)、α-乙酰香树脂醇(12)、达玛-24-烯-3β-十四酰氧基-20S-醇(13)、大戟烷-8,24-二烯-3-醇乙酸酯(14)和multiflorenylacetate(15)。结论 1～15均为首次从蒙古鸦葱中分离得到,其中2,3,5～15为首次从该属中分离得到。药理实验表明,在50mg.L-1质量浓度下,化合物1～5对人肺癌细胞A-549、14对人肝癌细胞株Bel-7402显示有意义的细胞毒活性。
Objective To study the anti-tumor activity of Radix Aconitum Methods The compounds were isolated and purified by silica gel, Sephadex LH-20, ODS and HPLC. The structures of the compounds were identified by their physico-chemical properties and spectral analysis. MTT assay and SRB method were used to determine the activity and the antitumor activity of monomeric compounds. Results Fifteen triterpenoids were isolated and identified as luteolin (1), betulin (2), betulinic acid (3), acetylsalicylic alcohol (4), 23Z-3β-acetoxyeupha- Damanesol acetate (6), oleanane acetate (7), dandelion sterol (8), dandelion sterol acetate (9), pseudo (10), pseudo dandelion sterol acetate (11), alpha-acetylenic alcohol (12), damma-24-ene-3 beta -tetradecanoxy-20S-alcohol Alkane-8,24-dien-3-ol acetate (14) and multiflorenylacetate (15). Conclusions 1 ~ 15 were isolated from Carambola for the first time, of which 2,3,5 to 15 were isolated from this genus for the first time. Pharmacological experiments showed that compounds 1 to 5 showed significant cytotoxic activity against human lung cancer cells A-549,14 and against human hepatoma Bel-7402 at a concentration of 50 mg.L-1.