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作者用聚丙交酯和乙交酯共聚物(PLG)制成微球包裹不同剂量的卵清蛋白(OVA).于0和4周用微球经口免疫雌性BALB/c 小鼠,初免和加强免疫均为连续口服3天.于初免后2、4、6和8周取小鼠唾液、肠道和阴道洗液及血清,用ELISA法测定IgA、IgG抗体.并于初免后1、5、9和13周取小鼠唾液腺和鼻粘膜组织.用酶联免疫斑点法(ELISPOT)检测抗体形成细胞(AFC).对照小鼠则口服可溶性抗原.结果显示,可溶性抗原初免或加强免疫后,血清中均未测得IgA抗体,IgG抗体可于第8周测得;初免含抗原微球后2周小鼠产生显著的血清IgA抗体应答,加强免疫后应答显著增强,8周时达最高峰,IgG抗体滴度也明显高于对照组.可溶性抗原初免后,可在小鼠唾液中检得IgA抗体,加强免疫后抗体滴度升高;而试验组小鼠2次免疫后的唾液IgA抗体均明显高于对照组.可溶性抗原免
The authors used microspheres made of polylactide and glycolide copolymer (PLG) to encapsulate different doses of ovalbumin (OVA). Female BALB / c mice were orally immunized with microspheres at 0 and 4 weeks, with prime and booster immunizations All patients were given orally for 3 consecutive days.The saliva, intestinal and vaginal washes and serum of mice were taken at 2, 4, 6 and 8 weeks after initial immunization, and IgA and IgG antibodies were measured by ELISA, And the mouse salivary glands and nasal mucosa tissues were taken at weeks 9 and 13. Antibody-forming cells (AFCs) were detected by ELISPOT and the control mice were orally administered with soluble antigens.The results showed that soluble antigens were immunoprecipitated or boosted , No IgA antibody was detected in serum and IgG antibody was measured at the 8th week. The serum IgA antibody response was significant at 2 weeks after primary immunization with antigen microspheres, and the response was significantly enhanced after booster immunization, reaching the peak at 8 weeks , IgG antibody titer was also significantly higher than that of the control group.After initial immunization of soluble antigen, IgA antibody was detected in the saliva of mice to enhance the antibody titer after immunization; while in the experimental group, the salivary IgA Antibodies were significantly higher than the control group