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应用ALC/GPC244系列全自动高效液相色谱仪定量测定了食糖类,果汁和酒类,水果和蔬菜类,乳及乳制品以及富含淀粉的谷类,豆类和薯类等55个样品中多种游离糖及淀粉水解糖的含量。 用果糖、葡萄糖、蔗糖、麦芽糖、乳糖、半乳糖、棉子糖、阿拉伯糖、山梨糖、木糖、甘露糖、蕈糖、鼠李糖和纤维二糖等共十四种糖做了分离和定量的测试条件。用不同比例的乙腈和水为流动相分别分离单、双糖,三糖及四糖。用果糖、葡萄糖、蔗糖、乳糖、半乳糖和棉子糖等6种糖做了重复测定试验;保留时间较长的蔗糖和棉子糖的重复测定结果的变异系数分别为3.2％和3.5％,其余均在3％以内。于14个样品中加入标准糖测定回收率(％),其平均值±标准差分别为:果糖97.8±6.4,葡萄糖100.0±9.8,蔗糖96.1±7.9,乳糖97.0±7.5,半乳糖96.4±4.58,麦芽糖107.6％,棉子糖91.7％。 高效液相色谱法应用于食物碳水化合物的测定具有简单、快速、准确而有特异性的优点,为测定食品中多种糖提供了较为可靠的分析方法。
ALC / GPC244 series of automatic high performance liquid chromatography quantitative determination of sugar, fruit juice and alcohol, fruits and vegetables, milk and dairy products and starch-rich cereals, beans and potatoes, 55 samples more Free sugars and starch hydrolyzed sugar content. A total of 14 sugars such as fructose, glucose, sucrose, maltose, lactose, galactose, raffinose, arabinose, sorbose, xylose, mannose, Quantitative test conditions. Different proportions of acetonitrile and water were used as the mobile phase to separate mono-, di-, trisaccharides and tetrasaccharides respectively. The results showed that the coefficient of variation (CV) of the repeated determination of sucrose and raffinose with longer retention time were 3.2% and 3.5%, respectively, with six kinds of sugars such as fructose, glucose, sucrose, lactose, galactose and raffinose. The rest are less than 3%. The average standard deviation of the addition of standard sugar in 14 samples was 97.8 ± 6.4 for fructose, 100.0 ± 9.8 for glucose, 96.1 ± 7.9 for sucrose, 97.0 ± 7.5 for lactose, 96.4 ± 4.58 for galactose, 107.6% maltose, raffinose 91.7%. The application of HPLC in the determination of food carbohydrates has the advantages of simple, rapid, accurate and specific, and provides a more reliable analytical method for determination of various sugars in food.