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目的:S100A16在肥胖以及多种恶性肿瘤发生发展中发挥了重要作用,本研究拟构建S100A16基因敲除小鼠模型。方法:利用基因表达调控系统(即Cre/lox P系统)构建全身敲除小鼠。采用聚合酶链式反应(PCR)鉴定小鼠的基因型,采用实时定量PCR(QRT-PCR)、Western blot方法验证其转录及翻译水平的表达。结果:成功建立了S100A16全身敲除小鼠模型,基因敲除杂合子小鼠成功饲养繁殖,目前为止未出现纯合子小鼠。S100A16敲除小鼠主要代谢器官,如脂肪、肌肉、肝脏中S100A16蛋白表达量显著下降。结论:S100A16基因敲除小鼠模型的构建为研究肥胖及胰岛素抵抗中的作用及机制提供了动物模型。
Objective: S100A16 plays an important role in the development of obesity and many malignant tumors. In this study, S100A16 knockout mouse model was proposed. Methods: Whole body knockout mice were constructed using the gene expression regulatory system (Cre / lox P system). The genotypes of mice were identified by polymerase chain reaction (PCR) and the transcription and translation levels were verified by real-time quantitative PCR (QRT-PCR) and Western blot. Results: The model of S100A16 knockout mice was successfully established. The knockout heterozygous mice were successfully fed and reproduced, and so far no homozygous mice were found. S100A16 knockout mouse major metabolic organs, such as fat, muscle, liver S100A16 protein expression decreased significantly. Conclusion: The construction of S100A16 knockout mouse model provides an animal model for studying the role and mechanism of obesity and insulin resistance.