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目的：弥补单纯珊瑚无骨诱导活性、骨修复能力弱等缺陷，给临床提供一种良好的骨移植替代材料。方法：将珊瑚与胶原和ｒｈＢＭＰ－２复合，制备出珊瑚／胶原／ｒｈＢＭＰ－２复合人工骨，将其植入大鼠背部肌肉陷窝内，以珊瑚／胶原和珊瑚／ｒｈＢＭＰ－２复合人工骨植入作对照；取材后通过组织学方法和图像分析评价其骨诱导活性。结果：珊瑚／胶原／ｒｈＢＭＰ－２植入后１周，在局部诱导出软骨细胞分化和软骨基质形成；４周形成含骨髓的板层骨；诱导成骨的量有明显的ｒｈＢＭＰ－２剂量依赖性（Ｐ＜０．０１）；而珊瑚／胶原和珊瑚／ｒｈＢＭＰ－２植入区均无骨或软骨形成。结论：珊瑚／胶原／ｒｈＢＭＰ－２复合人工骨具有良好的异位诱导成骨活性，是一种较理想的骨移植替代材料。
OBJECTIVE: To make up for the defects of pure coral, such as osteoinductive activity and weak ability of bone repair, to provide a good alternative material for bone transplantation. METHODS: Coral / collagen / rhBMP-2 composite artificial bone was prepared by compounding collagen with rhBMP-2 and implanted into the lacunae of the back muscles of rats. Coral / collagen and rhBMP-2 composite artificial bone Implantation as a control; After the material was drawn by histological methods and image analysis to evaluate the osteoinductive activity. RESULTS: One week after coral / collagen / rhBMP-2 implantation, chondrocyte differentiation and cartilage matrix formation were induced locally; bone marrow-containing lamellar bone was formed at 4 weeks; and the amount of osteoblasts induced by rhBMP-2 was significantly dose dependent (P <0.01). However, there was no bone or cartilage formation in the coral / collagen and coral / rhBMP-2 implants. CONCLUSION: Coral / collagen / rhBMP-2 composite artificial bone has good ectopic osteogenic activity and is an ideal substitute for bone graft.