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[目的]观察大黄素对原代培养小鼠肝细胞非酒精性脂肪变的影响。[方法]采用改良的胶原酶原位灌流法分离小鼠肝细胞,以含50%胎牛血清的1640培养液造成肝细胞脂肪变,加入不同浓度大黄素,检测培养上清液肝酶活性并观察细胞形态学变化。[结果]以50%胎牛血清的1640培养液培养的肝细胞明显脂肪变性,大黄素干预组培养上清液丙氨酸氨基转移酶(ALT)、门冬氨酸转氨酶(AST)、谷氨酰转肽酶(GGT)、乳酸脱氢酶(LDH)活性较模型组显著降低,苏木精-伊红染色显示肝细胞脂肪变性减轻。[结论]大黄素可明显减轻原代培养小鼠肝细胞的脂肪变性。
[Objective] To observe the effects of emodin on nonalcoholic steatosis in primary cultured mouse liver cells. [Methods] Mouse hepatocytes were isolated by in situ perfusion of modified collagenase, and hepatocyte steatosis was induced by 1640 medium containing 50% fetal bovine serum. Different concentrations of emodin were added to detect the liver enzyme activity of the culture supernatant. Observe changes in cell morphology. [Results] The hepatocytes cultured in 1640 medium with 50% fetal bovine serum showed marked steatosis, and the supernatants of the emodin intervention group were alanine aminotransferase (ALT), aspartate aminotransferase (AST), and glutamine. The activity of acyltransferase (GGT) and lactate dehydrogenase (LDH) was significantly lower than that of the model group. Hematoxylin-eosin staining showed a decrease in hepatic steatosis. [Conclusion] Emodin can significantly reduce the fatty degeneration of primary cultured mouse hepatocytes.