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目的探讨自体骨髓间充质干细胞(mMSCs)携带micro-dystrophin基因在移植鼠体内分化为有功能肌细胞的可能性。方法采用脂质体介导micro-dystrophin基因转染mdx小鼠mMSCs,通过尾静脉注射移植治疗mdx鼠,在移植后不同时间点对腓肠肌进行HE染色、计数核中心移位纤维(CNF)比例,并用免疫荧光法检测micro-dystrophin的表达。结果移植后各时间点腓肠肌病理改变较对照组有所改善,CNF比例下降,差异有统计学意义,部分肌细胞膜能表达micro-dystrophin蛋白,并随移植时间延长micro-dystrophin阳性肌纤维比例增加,分别达到3%(8周时)、6%(12周时)和8%(16周时)。结论自体mMSCs可携带外源性micro-dys-trophin基因在受体鼠体内分化为micro-dystrophin阳性肌细胞。
Objective To investigate the possibility of autologous bone marrow mesenchymal stem cells (mMSCs) carrying micro-dystrophin gene differentiated into functional muscle cells in transplanted mice. Methods mdx mouse mMSCs were transfected with liposome-mediated micro-dystrophin gene and mdx mice were transplanted by tail vein injection. The gastrocnemius muscles were stained with hematoxylin and eosin (HE) at different time points after transplantation and the ratio of CNFs was counted. The expression of micro-dystrophin was detected by immunofluorescence. Results The pathological changes of gastrocnemius were improved in all groups at each time point after transplantation compared with those in control group. The proportion of CNF decreased, the difference was statistically significant. The expression of micro-dystrophin protein in some muscle cell membrane and the increase of micro-dystrophin positive muscle fiber ratio Reached 3% (8 weeks), 6% (12 weeks) and 8% (16 weeks). Conclusion The autologous mMSCs can differentiate into micro-dystrophin positive muscle cells in the recipient mice by carrying exogenous micro-dys-trophin gene.