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建立了离子对高效液相色谱法分离与测定复方制剂（新灭菌）中的羟氨苄青霉素及氟氯青霉素含量法。采用Ｃ８．１０μｍ，４．６×２００ｍｍ柱（大连化物所Ｓｐｈｅｒｉｓｏｒｂ），以含１．４％十六烷基三甲基溴化铵的ｐＨ６．５磷酸盐缓冲液－乙腈（６８＋３２）为流动相，在紫外检测器２５４ｎｍ波长处检测，线性范围为０．２５～２ｍｇ／ｍＬ，ｒ＝０．９９９９（ｎ＝５），羟氨苄青霉素及氟氯青霉素平均回收率分别为９９．５％及１００．７％（ｎ＝５）。精密度：羟氨苄青霉素及氟氯青霉素日内（４ｈ）平均ＲＳＤ（相对标准偏差）均为０．８％（ｎ＝１５），日间（２ｈ）平均ＲＳＤ分别为０．６％和０．７％（ｎ＝３）。
A method for the separation and determination of amoxicillin and chlorfluralin in compound preparations (neo-sterilization) was established by ion-pair high performance liquid chromatography. Using a C8.10 μm, 4.6 × 200 mm column (Spherisorb, Dalian Institute of Chemical Industry) and a mobile phase of 1.4% hexadecyltrimethylammonium bromide in pH 6.5 phosphate buffer-acetonitrile (68 + 32) , With a linear range of 0.25-2 mg / mL at a wavelength of 254 nm at UV detector (r = 0.9999, n = 5). The mean recoveries of amoxicillin and chlorfluracycline were 99.5% and 100%, respectively .7% (n = 5). Precision: The mean RSD (n = 15) for both amoxicillin and flucillin during the day (4h) were 0.8% (n = 15), and the average RSD was 0.6% and 0.7 % (N = 3).