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应用聚合酶链反应(PCR)改良二次扩增法,检测48例鼻咽癌(NPC)患者石蜡包埋组织中的HPV-DNA,阳性率14.6％(7/48);与18例慢性鼻咽炎患者对照,其阳性率为0;差异有高度显著性(P<0.01)。同时检测EBV-DNA,NPC组阳性率为66.7％(32/48),对照组为27.8％(5/18)。两组差异亦有高度显著性(P<0.01)。7例HPV阳性患者中有6例EBV阳性,并且HPV阴性者均发生在低分化型NPC者中。故认为,HPV与EBV在NPC的病因学中可能有协同作用;因而可为临床监测NPC的分化程度及预后、治疗等提供依据;改良二次扩增法为检测HPV及EBV提供了一种更特异、更准确的新方法。
HPV-DNA in paraffin-embedded tissues from 48 patients with nasopharyngeal carcinoma (NPC) was detected by polymerase chain reaction (PCR) secondary amplification. The positive rate was 14.6% (7/48) Pharyngitis control patients, the positive rate was 0; the difference was highly significant (P <0.01). Meanwhile, the positive rate of EBV-DNA in NPC group was 66.7% (32/48) in NPC group and 27.8% (5/18) in control group. The difference between the two groups was also highly significant (P <0.01). Six of the seven HPV-positive patients were positive for EBV, and HPV-negative patients were all found in poorly differentiated NPCs. Therefore, HPV and EBV may play a synergistic role in the etiology of NPC, and thus provide a basis for clinical monitoring of NPC differentiation and prognosis, treatment, etc .; improved secondary amplification for the detection of HPV and EBV provides a more Specific, more accurate new method.