沙门菌是一种重要的食源性致病菌,引起全世界范围内的主要公共卫生问题。我们之前的研究已显示,在体内和体外感染模型中,葡萄糖抑制性分隔基因的缺失显著地改变了沙门菌的毒力。在大肠杆菌中,GidA和MnmE通过一种后转录机制修改tRNA而改变了某些细菌因子。因此,我们假设,在沙门菌中GidA和MnmE通过一种相似的机制共同调节毒力基因。为了验证我们的假设,并且检验GidA和MnmE在调节沙门菌毒力方面的相对作用,我们 Salmonella is an important food-borne pathogen that causes major public health problems worldwide. Our previous studies have shown that the absence of the glucose-suppressing spacer gene significantly alters the virulence of Salmonella in both in vivo and in vitro infection models. In E. coli, GidA and MnmE alter certain bacterial factors by modifying the tRNA by a post-transcriptional mechanism. Therefore, we hypothesized that in Salmonella GidA and MnmE regulate a virulence gene through a similar mechanism. In order to validate our hypothesis and examine the relative role of GidA and MnmE in the regulation of Salmonella virulence, we