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[Objective] The aim of this study is to evaluate the safety and bacteriostasis of Yandureqing (AEE) and its microemulsion (AEE-ME). [Method] The acute toxicity was tested in mice by intragastric administration, and median lethal dose (LD50) as well as its 95% confidence interval was calculated by modified Karber method; the bacteriestasis was investigated by cylinder plate method. [Result] LD50 of AEE in mice was 10.937 g/kg with the 95% confidence interval of 9.309-12.850 g/kg; and LD50 of AEE-ME in mice was 5.357 g/kg with the 95% confidence interval of 4. 388-6.566 g/kg. The MICs of AEE to Escherichia coli O149 from swine, Staphylococcus aureus, Salmonella pullorum and Streptococcus agalactiae were 10.00, 20.00, 20.00 and 10.00 mg/ml, respectively; while the MICs of AEE-ME to the 4 kinds of bacteria mentioned above were 5.00, 10.00, 5.00 and 5.00 mg/ml in turn, and that to Pseudomonas aeruginosa is 20.00 mg/ml. [Conclusion] AEE is an actually nontoxic drug and AEE-ME belongs to the low toxic preparation. AEE and AEE-ME have obvious bacteriostasis, in which AEE-ME is superior to AEE.