荧光素(Fluorescein)是重要的荧光试剂,在荧光分析中有广泛的应用。近来发现荧光素水溶液还可用于光分解水制氢,在光化学研究中也有重要意义。本文对荧光素水溶液的吸收光谱和荧光光谱进行了研究。荧光素水溶液在pH>8时其荧光发射在阳离子型表面活性剂溴代十六烷基三甲胺(CTAB)的临界胶团浓度(CMC)处有一个突变,而当溶液的pH<1时其荧光发射则在阴离子型表面活性剂十二烷基磺酸钠(SDS)的CMC处有很大的变化。而在相应的条件下荧光素水溶液的吸收光谱的变 Fluorescein is an important fluorescent reagent and has been widely used in fluorescence analysis. Recently, it has been found that fluorescein aqueous solution can also be used for photolysis of hydrogen to produce water, which is also of great significance in photochemical studies. In this paper, the absorption and fluorescence spectra of fluorescein aqueous solution were studied. Fluorescence emission of fluorescein solution at pH> 8 has a mutation at the critical micelle concentration (CMC) of the cationic surfactant bromocetyl trimethylamine (CTAB). When the solution pH is less than 1 Fluorescence emission is greatly changed at the CMC of the anionic surfactant sodium dodecyl sulfate (SDS). Under the corresponding conditions, the absorption spectrum of fluorescein aqueous solution changes