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目的研究岩黄连Corydalis saxicola生物碱中具有抗乙肝病毒活性的化学成分。方法利用柱色谱法分离纯化岩黄连总生物碱中的化学成分,根据化合物的理化性质与波谱数据进行结构鉴定;利用HepG 2.2.15细胞株对质量分数较高的化学成分进行抗乙肝病毒活性试验。结果从岩黄连总生物碱中分离鉴定了16个生物碱,分别为二氢血根碱(1)、d-紫堇碱(2)、旋卡文定碱(3)、stylopine(4)、6-丙酮基-5,6-二氢血根碱(5)、二氢白屈菜红碱(6)、四氢巴马汀(7)、adlumidine(8)、(-)-salutaridine(9)、巴马汀(10)、原阿片碱(11)、小檗碱(12)、coptisine(13)、thalifaurine(14)、dehydroapocavidine(15)和木兰花碱(16)。对质量分数较高的化合物5、6、8～11、13、16进行抗乙肝病毒活性试验,结果表明化合物5、8活性一般,化合物11、16活性较弱,化合物6对HBV具有较强的抑制作用,化合物9次之。结论化合物1、4～6、8、9、13、14和16均为首次从该药用植物分离得到,化合物10的质量分数较高,是该植物的主要成分,化合物6对HBV抑制活性较强,可能是该植物主要的活性成分。
Objective To study the chemical constituents of Corydalis saxicola alkaloids with anti-hepatitis B virus activity. Methods The constituents were isolated and purified by column chromatography, and their chemical structures were elucidated on the basis of the physicochemical properties and spectral data of the compounds. HepG 2.2.15 cells were used to test the anti-hepatitis B virus activity of chemical constituents with high mass fraction . Results Sixteen alkaloids were isolated and identified from the total alkaloids of Rhizoma Coptidis, namely dihydroquinate (1), d-corydaline (2), cyclavasamine (3), stylopine (5), dihydroquercetin (6), tetrahydropalmatine (7), adlumidine (8), (-) - salutaridine ), Palmatine (10), protopine (11), berberine (12), coptisine (13), thalifaurine (14), dehydroapocavidine (15) and magnoliaine (16). The anti-HBV activities of compounds 5, 6, 8-11, 13 and 16 with higher mass fraction were tested. The results showed that compounds 5 and 8 were active and compounds 11 and 16 were weakly active. Compound 6 showed strong activity against HBV Inhibition, compound 9 times. Conclusions Compounds 1, 4 ~ 6, 8, 9, 13, 14 and 16 are isolated from this medicinal plant for the first time. Compound 10 has the highest mass fraction and is the main component of this plant. Strong, may be the main active ingredient of the plant.