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目的:探讨KAI1在胃癌中的表达情况及其与胃癌侵袭、转移和预后的关系。方法:以52例胃癌患者为对象,分别应用RT-PCR、蛋白印迹、免疫组化方法检测KAI1在胃癌、癌旁正常胃黏膜及淋巴结转移癌中的表达;对所有患者进行随访,分析KAI1与胃癌侵袭、转移和预后的关系。结果:KAI1在正常胃黏膜中多数为中度表达,而在胃癌和淋巴结转移癌中多数表达减弱或缺失,在低分化癌、黏液腺癌中表现尤为明显,差异有统计学意义(P<0.05)。其mRNA和蛋白质表达水平具有较高的一致性。TNM分期晚者(T4,N3)胃癌中KAI1表达明显减低。KAI1高表达组患者5年生存率为68.8%,而低表达组为29.6%,差异有统计学意义(P=0.0133)。结论:KAI1表达与胃癌的侵袭、转移和预后关系密切,是反映胃癌恶性生物学行为较好的分子指标。
Objective: To investigate the expression of KAI1 in gastric cancer and its relationship with invasion, metastasis and prognosis of gastric cancer. Methods: Fifty-two patients with gastric cancer were enrolled in this study. The expression of KAI1 in gastric cancer, adjacent normal gastric mucosa and lymph node metastasis was detected by RT-PCR, Western blotting and immunohistochemistry. All patients were followed up and analyzed for KAI1 and Relationship between gastric cancer invasion, metastasis and prognosis. Results: Most of KAI1 was moderately expressed in normal gastric mucosa, while most of them were decreased or absent in gastric cancer and lymph node metastasis, especially in poorly differentiated carcinoma and mucinous adenocarcinoma, with significant difference (P <0.05 ). Its mRNA and protein expression levels have high consistency. The expression of KAI1 was significantly decreased in gastric cancer with TNM staging (T4, N3). The 5-year survival rate was 68.8% in patients with KAI1 overexpression compared with 29.6% in patients with low expression (P = 0.0133). Conclusion: The expression of KAI1 is closely related to the invasion, metastasis and prognosis of gastric cancer, which is a good molecular indicator of malignant behavior of gastric cancer.