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目的:1994~1997年间作者对福建宁化已发现的斑点热疫区内进行蜱类及宿主感染调查。方法:分别在鼠类、野兔、野猪、麂、狗、牛等宿主体表捕捉各种蜱类,用鸡胚卵黄囊感染法分离斑点热群立克次体,用微量室温补体结合、免疫萤光及PCR/RFLP技术对分离株进行鉴定。结果:分类鉴定5000余只蜱类共5属13种。麂、鼠类、野猪斑点热群立克次体感染率分别为12.50%,9.47%,58.33%。分别从野兔、麂体表寄生的越原血蜱中分离出斑点热群立克次体。结论:从媒介、宿主、病原学上进一步证实福建存在北亚斑点热疫源地的可能性。
OBJECTIVE: From 1994 to 1997, the authors investigated ticks and host infections in the plague-endemic areas that have been found in Ninghua, Fujian Province. Methods: Various ticks were captured on the body surface of rodents, rabbits, wild boars, muntjac, dogs and cattle respectively. Rickettsia rickettsis was isolated by chick embryo yolk sac infection. Isolates were identified by light and PCR / RFLP techniques. Results: More than 5000 species of ticks were identified in 5 genera and 13 species. The rates of rickettsia in deer, rodents and boar spotted fever group were 12.50%, 9.47% and 58.33%, respectively. Species of Rickettsia giganteum were isolated from the wild blood-sucking ticks of hare and otter body respectively. CONCLUSION: The possibility of existence of northern Asian spot fever in Fujian Province is further confirmed by the media, host and etiology.