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本文对经ＳｉＯ＿２体内暴露４～５周的矽肺大鼠肺泡巨噬细胞（ＡＭ）上清中促纤维母细胞（ＦＢ）生长的物质进行了分离纯化。由高压液相层析（ＨＰＬｃ）分析结果表明，与正常对照大鼠ＡＭ相比。矽肺大鼠ＡＭ在经体外６ｈ的无血清培养后，上清中含有两个组分的促ＦＢ生长的活性组分。第一组分位于分子量约３５～６０ＫＤａ区间，保留时间为７～９ｍｉｎ，有轻度的促ＦＢ生长活性和ＩＬ－１及ＴＮＦ活性；第二组分位于分子量６～９ＫＤａ区间，保留时间为１２～１４ｍｉｎ，有明显的促ＦＢ生长活性。但不具有任何可检测的ＩＬ－１和ＴＮＦ活性，提示矽肺状态下ＡＭ可能主要通过这种非ＩＬ－１和ＴＮＦ样物质的大量释放而促进ＦＢ的增生，并进而导致肺间质的广泛纤维化。
In this paper, we isolated and purified the fibroblast (FB) growth substances in the supernatant of alveolar macrophages (AM) of silicotic rats exposed to SiO 2 for 4 to 5 weeks in vivo. Analysis by high pressure liquid chromatography (HPLc) showed that compared to normal control rats AM. Silicosis rat AM after 6h in vitro serum-free culture, the supernatant contains two components of the FB-promoting active ingredients. The first component is located in the molecular weight of about 35 ~ 60KDa interval retention time of 7 ~ 9min, with a slight promotion of FB growth activity and IL-1 and TNF activity; the second component is located in the molecular weight of 6 ~ 9KDa interval, retention time of 12 ~ 14min, there is a clear promotion of FB growth activity. But did not have any detectable IL-1 and TNF activity, suggesting that AM may promote FB hyperplasia mainly through the massive release of this non-IL-1 and TNF-like substance in the silicotic state and thus lead to extensive interstitial fibrosis The