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目的探讨Toll样受体7(TLR7)和NF-κB在食管鳞癌组织中的表达及其临床意义。方法采用实时荧光定量PCR(qRT-PCR)法检测20例食管鳞癌及相应癌旁正常组织中TLR7和NF-κB mRNA的表达;采用免疫组织化学方法检测90例食管鳞癌及相应癌旁正常组织中TLR7和NF-κB蛋白的表达。结果 (1)食管鳞癌组织中TLR7和NF-κB mRNA的表达量为(0.72±0.59)、(0.69±0.32),显著高于癌旁正常组织的(0.45±0.36)、(0.47±0.16),其表达差异均有统计学意义(P<0.05)。(2)食管鳞癌组织中TLR7和NF-κB蛋白的阳性表达率为77.6%(68/90)、75.4%(67/90),明显高于癌旁正常组织的18.9%(17/90)、33.3%(30/90),其表达差异均有统计学意义(P<0.05);TLR7蛋白表达与肿瘤的分化程度和肿瘤浸润深度密切相关,表达差异均有统计学意义(P<0.05);NF-κB蛋白(p65)表达与肿瘤的分化程度和淋巴结转移密切相关,表达差异均有统计学意义(P<0.05)。(3)食管癌组织中TLR7和NF-κB蛋白的表达呈正相关(r=0.259,P=0.014)。结论 TLR7可能通过上调NF-κB mRNA和蛋白的表达,促进食管鳞癌的发生与发展。
Objective To investigate the expression of Toll-like receptor 7 (TLR7) and NF-κB in esophageal squamous cell carcinoma and its clinical significance. Methods The expression of TLR7 and NF-κB mRNA in 20 cases of esophageal squamous cell carcinoma and adjacent normal tissues were detected by real-time quantitative PCR (qRT-PCR). The expressions of TLR7 and NF-κB mRNA were detected by immunohistochemistry in 90 esophageal squamous cell carcinomas and corresponding paracancerous normal Tissue TLR7 and NF-κB protein expression. Results (1) The expressions of TLR7 and NF-κB mRNA in esophageal squamous cell carcinoma were (0.72 ± 0.59) and (0.69 ± 0.32), respectively, which were significantly higher than those in normal tissues (0.45 ± 0.36 and 0.47 ± 0.16) , The difference was statistically significant (P <0.05). (2) The positive rates of TLR7 and NF-κB in esophageal squamous cell carcinoma were 77.6% (68/90) and 75.4% (67/90), respectively, which were significantly higher than those in normal tissues (18.9%, 17/90) , 33.3% (30/90), respectively. The expression of TLR7 protein was closely related to tumor differentiation and tumor invasion depth (P <0.05) The expression of NF-κB protein (p65) was closely related to the degree of tumor differentiation and lymph node metastasis, the differences were statistically significant (P <0.05). (3) The expression of TLR7 and NF-κB protein in esophageal cancer was positively correlated (r = 0.259, P = 0.014). Conclusion TLR7 may promote the occurrence and development of esophageal squamous cell carcinoma by up-regulating the expression of NF-κB mRNA and protein.