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1977年,Asch,Fernandez 及 Pauerstein 报道,用荧光免疫技术证明,免疫兔产生的 HCG 抗血清能与空气干燥的人类的死精子发生反应,提示在精子细胞上存在着 HCG 样的物质。本文试图确定,用免疫学技术是否能检定出在人类的活精子表面存在某种 HCG 样的物质。从免疫兔及正常兔分别得到 HCG 抗血清及正常兔血清,离心分离出血清后,再离心移除红细胞,在56℃加热,然后放于-20℃贮存。从健康男性志愿者得到正常人血清。用豚鼠血清价为补体。本实验中所用精子系采自23名不同男人射出的
In 1977, Asch, Fernandez and Pauerstein reported that immunofluorescence showed that the antiserum produced by immunized rabbits reacted with dead sperm in air-dried humans, suggesting the presence of HCG-like substances on sperm cells. This article attempts to determine whether immunological techniques can detect the presence of certain HCG-like substances on the surface of human live sperm. HCG antiserum and normal rabbit serum were obtained from the immunized rabbits and normal rabbits, respectively. Serum was separated by centrifugation, then red blood cells were removed by centrifugation, heated at 56 ℃ and stored at -20 ℃. Normal human serum is obtained from healthy male volunteers. Guinea pig serum price of complement. The sperm used in this experiment was collected from 23 different men