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以表达融合蛋白的载体PUR288以及高效表达载体PBV221作载体,在大肠杆菌内成功地表达了我国克隆的HPV6BVL_I基因,并对表达蛋白进行免疫学鉴定,对两种载体的表达情况进行了讨论。
The fusion protein vector PUR288 and high expression vector PBV221 were used as vectors to express HPV6BVL_I gene cloned successfully in E. coli and the expressed proteins were identified by immunology. The expression of the two vectors was discussed.