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应用聚合酶链反应(PCR)技术检测30例鼻咽癌患者的鼻咽活检组织中EB病毒DNA,以17例慢性扁桃体炎、鼻咽炎、慢性咽炎及颈淋巴结核患者的组织作对照。全部病例均行病理切片检查。结果:30例鼻咽癌活检组织中,EB病毒DNA 阳性28例(93.33%),阴性2例(6.66%)。对照组17例均呈阴性。说明:应用鼻咽部活检组织进行PCR反应检测EB病毒DNA,是一种方法简单、特异性强、灵敏度高的检测手段。对鼻咽癌早期发现、早期诊断与早期治疗具有重要的临床意义。
Polymerase chain reaction (PCR) was used to detect Epstein-Barr virus DNA in nasopharyngeal biopsies from 30 patients with nasopharyngeal carcinoma. Twenty-seven patients with chronic tonsillitis, nasopharyngitis, chronic pharyngitis and cervical lymph node tuberculosis were used as controls. All cases underwent biopsy. Results: Among the 30 cases of nasopharyngeal carcinoma, EBV DNA was positive in 28 cases (93.33%) and negative in 2 cases (6.66%). Control group, 17 cases were negative. Description: The application of nasopharyngeal biopsy tissue PCR detection of Epstein-Barr virus DNA, is a simple, specific and sensitive detection means. Early detection of nasopharyngeal carcinoma, early diagnosis and early treatment has important clinical significance.