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目的:建立以苯酚-硫酸法测定清开灵注射液中总多糖含量的方法。方法:以苯酚-硫酸法显色,以葡萄糖为对照品,测定清开灵注射液中总多糖的含量。结果:葡萄糖的检测浓度在5.037~125.9μg.mL-1范围内与吸光度呈良好的线性关系(r=0.9984);平均回收率为100.02%,RSD=2.30%(n=6)。结论:该方法简单、准确、重复性好,可用于清开灵注射液中总多糖的含量测定。
Objective: To establish a method for determination of total polysaccharide in Qingkailing injection by phenol-sulfuric acid method. Methods: The phenol-sulfuric acid method was used for color development. Glucose was used as the reference substance to determine the content of total polysaccharide in Qingkailing injection. Results: The detection concentration of glucose in the range of 5.037-125.9 μg.mL-1 showed a good linear relationship with the absorbance (r=0.9984); the average recovery was 100.02%, and the RSD was 2.30% (n=6). Conclusion: This method is simple, accurate and reproducible. It can be used for the determination of total polysaccharide in Qingkailing injection.