论文部分内容阅读
目的:探讨益气活血解毒方对经皮血管腔内成形术(PTA)后再狭窄的防治作用。方法:采用电刺激兔颈总动脉加高脂饲料8周后,体表多普勒超声评价颈动脉粥样斑块狭窄,再行颈动脉PTA,形成再狭窄。造模后动物随机分为模型组、西药组(普伐他汀和巴米尔)、中药组(益气活血解毒方),另设正常组和假手术组作为对照,含药饲料治疗4周。分别在不同时间点检测血脂、血清C-反应蛋白(CRP)、转化生长因子(TGF-β)。药后4周颈总动脉取材,常规HE、弹力+VG和Masson染色,平滑肌肌动蛋白(α-SM-actin)和巨噬细胞RAM11免疫组化分析,所有病理切片经显微图像分析系统分析。结果:益气活血解毒方突出表现为对增生内膜中胶原的抑制作用,减少狭窄局部胶原面积比达50%;能减少血清中TGF-β的含量,降低血脂;而对血清CRP、增生内膜中巨噬细胞RAM11的表达无明显抑制作用。结论:益气活血解毒方通过减少增生内膜中胶原的堆积、降低血脂和TGF-β的含量,防治PTA后再狭窄。
Objective: To investigate the preventive and therapeutic effects of Yiqi Huoxue Jiedu Decoction on restenosis after percutaneous transluminal angioplasty (PTA). METHODS: Eight weeks after electrostimulation of rabbit common carotid arteries with high-fat diet, surface Doppler ultrasonography was used to evaluate carotid atherosclerotic plaque stenosis, followed by carotid PTA and restenosis. After modeling, animals were randomly divided into model group, western medicine group (Pravastatin and Bamil), Chinese medicine group (Yiqi Huoxue Jiedu Fang), normal group and sham operation group as control, drug-containing feed treatment for 4 weeks. Blood lipids, serum C-reactive protein (CRP) and transforming growth factor (TGF-β) were measured at different time points. 4 weeks after drug administration, the common carotid artery was obtained, routine HE, elastic +VG and Masson staining, smooth muscle actin (α-SM-actin) and macrophage RAM11 immunohistochemical analysis, all pathological sections were analyzed by microscopic image analysis system. . Results: Yiqi Huoxue Jiedu Decoction showed prominent inhibition of collagen in hyperplastic intima, reduced local collagen area ratio of stenosis by 50%, reduced TGF-β content in serum, reduced blood lipid, and increased serum CRP, hyperplasia There was no significant inhibition of macrophage RAM11 expression in the membrane. Conclusion: Yiqi Huoxue Jiedu Decoction can prevent post-PTA restenosis by reducing the accumulation of collagen in hyperplastic intima, reducing blood lipid and TGF-β content.