论文部分内容阅读
甲基丙烯酸缩水甘油酯（GMA）为单体，二乙烯基苯（DVB）和三聚异氰尿酸三烯丙酯（TAIC）为交联剂，在致孔剂甲苯和正庚烷存在下，直接以Φ4．6×100mm色谱柱管为模具，通过原位聚合制备了聚（甲基丙烯酸缩水甘油酯-二乙烯基苯-三聚异氰尿酸三烯丙酯）（PGDT）型连续床．然后，利用二乙胺和骨架结构中的环氧基反应得到阴离子交换型连续床．对连续床的化学结构、孔结构及其对蛋白质的分离性能进行了研究．实验结果表明，连续床内部含有大量类似渠道的大孔，孔径为1～2μm．在流速高达3250cm／h时，背压仅为9．89Mpa．而且流速对色谱分离效率的影响小，高流速下仍能得到高分离效率，可以通过提高流速实现蛋白质的快速分离．
Glycidyl methacrylate (GMA) as a monomer, divinylbenzene (DVB) and triallyl cyanurate (TAIC) as the crosslinking agent, in the presence of porogen toluene and n-heptane, Poly ((glycidyl methacrylate - divinylbenzene - triallyl isocyanurate) (PGDT) continuous bed was prepared by in - situ polymerization using a Φ4.6 × 100mm column as a mold. The anion exchange type continuous bed is then obtained by reacting diethylamine with the epoxy groups in the backbone structure. The chemical structure, pore structure and the separation performance of protein in continuous bed have been studied. The experimental results show that the continuous bed contains a large number of similar channels of macropores, pore size of 1 ~ 2μm. In the flow rate up to 3250cm / h, the back pressure is only 9.89Mpa. Moreover, the effect of flow rate on chromatographic separation efficiency is small, high separation efficiency can still be obtained under high flow rate, and rapid protein separation can be realized by increasing flow rate.