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近年来在工业有用微生物如酵母、丝状真菌及细菌的原生质体融合方面有许多报道,但在食用蘑菇方面,主要由于缺乏对杂种选择的遗传或表型标记,还没有报道过种间或属间原生质体融合的例子,尽管有一些关于担子菌原生质体形成和再生的报告。因此,我们先由糙皮侧耳和鲑色侧耳的单核菌株分离营养缺陷突变体,然后研究彼此性不相容的这两个侧耳种间原生质体融合。本文报道用聚乙二醇(PEG)处理后分离融合子和融合子的同功酶分析结果。本试验用的单核菌株来自糙皮侧耳909和鲑色侧耳01的野生型双核体。这些菌株保存于PDA培养基上。原生质体形成方法:将菌培养于GMY培养基(10克葡萄糖、10克麦芽抽提物、4克酵母抽提物)上2～3天,收获菌丝用0.7M甘露醇洗
There have been many reports of protoplast fusion of industrially useful microorganisms such as yeasts, filamentous fungi and bacteria in recent years, but in the edible mushrooms, the lack of genetic or phenotypic markers for selection of hybrids has not been reported Examples of protoplast fusion, although there are some reports on the protoplast formation and regeneration of Basidiomycetes. Therefore, we first isolated auxotrophic mutants from monospora strains of Pleurotus ostreatus and Salmonella typhimurium, and then studied the protoplast fusion between these two Pleurotus species that are sexually incompatible with each other. This paper reports the results of isoenzyme analysis of the fusion and the fusion subunit after treatment with polyethylene glycol (PEG). The mononuclear strains used in this experiment were derived from the wild-type dinuclei of Pleurotus ostreatus 909 and Pleurotus ostreatus 01. These strains are stored on PDA medium. Protoplast formation method: The bacteria were cultured on GMY medium (10 g glucose, 10 g malt extract, 4 g yeast extract) for 2 to 3 days, and the mycelia harvested were washed with 0.7 M mannitol