小麦与玉米杂交产生的单倍体植株无染色体自然加倍发生 ,为获得加倍单倍体 ,进行了两种秋水仙素加倍处理。结果表明 ,在培养基中直接加入秋水仙素处理已培养 7d的幼胚 ,无秋水仙素对照的幼胚萌发率为 6 7.4 % ,存活的植株均未结实 ;而经过浓度为 5 0mg/L、10 0mg/L和 2 0 0mg/L秋水仙素处理的幼胚萌发率分别是 32 .1%、2 6 .4 %和 16 .3% ,加倍率分别是 85 .7%、10 0 %和 5 0 %。秋水仙素处理时间在 2 4～ 72h范围内 ,随着处理时间的延长 ,幼胚萌发率随之降低 ,死亡率随之增加 ,以 2 4h效果较好。用浓度为 5 0 0mg/L、75 0mg/L、10 0 0mg/L的秋水仙素溶液浸根加倍处理 ,平均加倍率分别是 89.6 %、76 .0 %和 73.3% ,而用浓度为 5 0 0mg/L秋水仙素处理壮苗获得了98.2 %的加倍率和 93.2 %的加倍处理效率 Chromosomes from haploid plants produced by the cross of wheat and maize naturally doubled and, in order to obtain doubled haploid, two colchicine doublings were performed. The results showed that immature embryos cultured for 7 days were directly treated with colchicine, the germination rate of immature embryos without colchicine control was 6 7.4%, and none of the surviving plants were stubble. However, after the concentration of 50 mg / L , The germination rates of immature embryos treated with 10 0 mg / L and 200 mg / L colchicine were 32.1%, 26.4% and 16.3%, respectively. The doubling rates were 85.7% and 100% And 50%. Colchicine treatment time in the 2-4 ~ 72h range, with the extension of treatment time, the immature embryo germination rate decreases, the mortality rate increases, with a good effect of 2 4h. Treated with colchicine solution of 500 mg / L, 75 0 mg / L and 100 mg / L, the average doubling rates were 89.6%, 76.0% and 73.3%, respectively. 0 0mg / L colchicine treatment of seedlings to obtain a 98.2% doubling rate and 93.2% doubling treatment efficiency