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目的:观察隔姜灸神阙、关元对类寒湿凝滞型原发性痛经(PD)大鼠内分泌相关分子及其受体基因表达的影响,探讨其镇痛机制.方法:将32只雌性Wistar大鼠根据随机数字表法分为正常组、模型组、隔姜灸组和西药组,每组8只.除正常组外,其余各组使用缩宫素联合冰水浴法建立类寒湿凝滞型PD大鼠模型.造模成功后,正常组和模型组不接受治疗;隔姜灸组接受隔姜灸神阙和关元治疗;西药组接受布洛芬灌胃治疗.比较各组大鼠扭体反应情况,检测血清中前列腺素F2α(PGF2α)、雌激素(E2)、孕激素(P)含量及子宫组织中PGF2α受体mRNA、E2受体mRNA表达.结果:正常组未出现扭体行为;与正常组比较,模型组血清PGF2α、E2含量增加(P<0.01),P含量下降(P<0.01);子宫PGF2α受体mRNA、E2受体mRNA表达增加(P<0.01,P<0.05).与模型组比较,隔姜灸组、西药组扭体行为潜伏期延长,扭体反应评分降低(P<0.01);隔姜灸组、西药组血清PGF2α、E2含量下降,P含量上升(P<0.05 or P<0.01);隔姜灸组、西药组子宫PGF2α受体mRNA、E2受体mRNA表达减少(P<0.05).与西药组比较,隔姜灸组扭体行为潜伏期延长,扭体反应评分降低(P<0.05),血清E2含量下降(P0.05).结论:隔姜灸对类寒湿凝滞型PD的镇痛效应可能与调控子宫组织中PGF2α受体mRNA及E2受体mRNA表达水平有关.“,”Objective: To observe the effects of ginger-partitioned moxibustion at Shenque (CV8) and Guanyuan (CV4) on the expression levels of endocrine-related molecules and their receptors in rats with primary dysmenorrhea (PD) due to cold-dampness stagnation, thus to explore their analgesic mechanisms. Methods: Thirty-two female Wistar rats were divided into a normal group, a model group, a ginger-partitioned moxibustion group, and a Western medicine group according to the random number table method, with 8 rats in each group. Except for rats in the normal group, all other rats were treated with oxytocin combined with ice-water bath to establish the rat models of PD due to cold-dampness stagnation. After successful modeling, rats in the normal group and the model group did not receive treatment; rats in the ginger-partitioned moxibustion group received treatments with ginger-partitioned moxibustion at Shenque (CV8) and Guanyuan (CV4); rats in the Western medicine group received ibuprofen by intragastric administration. The writhing response of rats was compared among groups, and the serum levels of prostaglandin F2α (PGF2α), estrogen (estradiol, E2), progesterone (P), and the mRNA expression of PGF2α and E2 receptors in the uterine tissues were detected. Results: No writhing behavior was observed in the normal group; compared with the normal group, the serum PGF2α and E2 levels in the model group were increased (P<0.01), while the P level was decreased (P<0.01), and the mRNA expression levels of the uterine PGF2α and E2 receptors were increased (P<0.01, P<0.05). Compared with the model group, the writhing behavior latency was prolonged, and the writhing response score was decreased in the ginger-partitioned moxibustion group and the Western medicine group (P<0.01); the serum PGF2α and E2 levels in the ginger-partitioned moxibustion group and the Western medicine group were decreased, while the P level was increased (P<0.05 or P<0.01); the mRNA expression levels of the uterine PGF2α and E2 receptors in the ginger-partitioned moxibustion group and the Western medicine group were decreased (P<0.05). Compared with the Western medicine group, the ginger-partitioned moxibustion group showed a prolonged writhing behavior latency, reduced writhing response score (P<0.05), and decreased serum E2 level (P0.05).Conclusion: The analgesic effect of ginger-partitioned moxibustion on PD due to cold-dampness stagnation may be related to regulating the mRNA expression levels of PGF2α and E2 receptors in the uterine tissues.