Experimental work was conducted at two apiaries located in Irbid district and in Shuna North, Jordan, during the years 2004-2006. The aims of these investigations were to estimate the seasonal changes in the infestation rates of the bee louse (Braula sp.) and to develop an easy and rapid method of estimating the infestation rate on workers with bee Braula. Two major honey bee subspecies are reared in Jordan; Apis mellifera cica and Apis mellifera syriaca were used in this study. The results showed that the infestation rate began to increase rapidly in May, reaching the season’s maximum rate of 16.2%, 15.8% and 17.4% forA. ra. cica and 22.6%, 23.9% and 22.9% forA. m. syr/aca in December of 2004,2005 and 2006, respectively. The maximum adult numbers of bees were found in April and June, whereas the minimum for the year was in January in both honey bee subspecies colonies during the study period. The actual population of the bee louse could be estimated by counting the daily dropped lice and multiplying by a factor of 158. This factor is valid for the experimental colonies of both subspecies kept for 3 years under semi-arid Mediterranean conditions.