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目的探讨抗辐灵对微波辐射致大鼠精子损伤的防治作用。方法 100只Wistar雄性大鼠,随机分为:正常对照组、辐射对照组、1 g/(kg.d)抗辐灵组、2 g/(kg.d)抗辐灵组和4 g/(kg.d)抗辐灵组,30 mW/cm2微波全身辐射10 min,于辐射前7 d灌胃给予1、2和4 g/(kg.d)抗辐灵水溶液,每天1次,连续14 d。分别于停药后6 h(辐射后7 d)、7 d(辐射后14 d)、14 d(辐射后21 d)处死大鼠,SCA精子动态分析系统检测大鼠精子活力参数,HE染色观测精子畸形率。结果 30mW/cm2微波辐射后7 d,大鼠精子畸形率升高(P<0.05);停药后6 h各剂量药物组精子畸形率较辐射组明显降低(P<0.05或P<0.01)。30 mW/cm2微波辐射后14 d大鼠精子活动率(AR)和A+B级精子比例明显减少(P<0.05),D级精子比例明显增加(P<0.05),精子畸形率增加(P<0.05)。停药后7 d;2和4 g/(kg.d)抗辐灵组精子活动率(AR)和A+B级精子比例较辐射组明显增加(P<0.05或P<0.01),D级精子比例降低(P<0.05),精子畸形率降低(P<0.05)。结论 30 mW/cm2微波辐射可引起大鼠精子损伤,表现为精子活动率减少和精子畸形率增加,抗辐灵具有明显防治微波辐射损伤的作用。
Objective To investigate the preventive and therapeutic effects of Kang Ling on the sperm damage induced by microwave in rats. Methods 100 Wistar male rats were randomly divided into normal control group, radiation control group, 1 g / (kg.d) Kanglu Ling group, 2 g / (kg.d) Kanglu Ling group and 4 g / kg.d) anti-Ling Ling group, 30 mW / cm2 microwave radiation for 10 min, 7 d before radiation intragastric administration of 1, 2 and 4 g / (kg.d) Radix solution, once daily for 14 d. Rats were sacrificed at 6 hours (7 days after irradiation), 7 days (14 days after irradiation) and 14 days (21 days after irradiation) respectively. The sperm motility parameters of rats were detected by SCA sperm dynamic analysis system. HE staining Sperm deformity rate. Results At the 7th day after 30mW / cm2 microwave irradiation, the sperm deformity rate increased (P <0.05). The sperm deformity rate of each dose group decreased significantly (P <0.05 or P <0.01) 6 h after drug withdrawal. Sperm motility (AR) and A + B sperm proportion decreased significantly (P <0.05), D grade sperm increased significantly (P <0.05) and sperm deformity rate increased after 30 mW / cm2 microwave radiation <0.05). The sperm motility (AR) and A + B sperm ratio of Radix Rehmanniae groups were significantly increased (P <0.05 or P <0.01) at 2 and 4 g / (kg · d) Sperm reduced (P <0.05), sperm deformity rate decreased (P <0.05). Conclusion 30 mW / cm2 microwave radiation can cause sperm damage in rats, showing a decrease of sperm motility and an increase of sperm deformity rate. Radix Rehmanniae has the obvious effect of preventing and treating microwave radiation injury.