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通过利用肝癌病人体内血清中所含的对肿瘤抗原产生的特异性抗体筛选肝癌组织cDNA表达文库的方法 (SEREX) ,筛选得到了可以诱导肝癌病人抗体免疫应答的两个新抗原HCA5 19基因(GenBankAF14 6 731)及其变异体HCA90基因 (GenBankAF 2 872 6 5 ) .它们定位于染色体 2 0q11 2 ,HCA5 19含 18个外显子 ,HCA90含 19个外显子 .其中HCA90所特有的外显子序列长 10 8bp ,属Alu重复序列片段 ,插入于HCA5 19外显子 10和 11之间 ,原为HCA5 19内含子序列 .该插入片段位于HCA5 19开放阅读框架之内 ,不改变HCA5 19的读码框 ,使HCA5 19编码的 74 7个氨基酸增长至HCA90的 783个氨基酸 .通过Northern杂交和RT PCR分析发现 ,HCA5 19和HCA90基因分别在 9 9例和 6 9例肝癌组织中高表达 ,RT PCR显示 ,它们在正常肝组织和其它正常组织中有极低水平转录本表达 .而这种低表达转录本在Northern杂交中不能被检测到 .HCA5 19蛋白被首次发现在肿瘤病人中能够诱导机体的抗体免疫应答 ,为一个新的肿瘤相关性抗原分子 .其变异体HCA90抗原基因为首次发现的新基因 .其功能可能与细胞的恶性增殖相关 ,并可进一步研究其作为临床肿瘤治疗和诊断的靶分子的可行性
Two novel antigens HCA5 19 gene (GenBank AF14, GenBank AF14, GenBank AF14, GenBank AF14, GenBank AF14, GenBank AF14, GenBank AF14, GenBank AF14 6 731) and its variant HCA90 gene (GenBankAF 2 872 6 5). They are located on chromosome 20q11 2, HCA5 19 contains 18 exons, and HCA90 contains 19 exons, of which HCA90 is characterized by exon The sequence is 10 8 bp in length and belongs to the Alu repeat sequence inserted between the exon 10 and 11 of HCA5 19 originally intron HCA519.The insert is located within the HCA5 19 open reading frame and does not alter the expression of HCA5 19 The 747 amino acids encoded by HCA5 19 increased to 783 amino acids in HCA90. Northern blot and RT PCR showed that HCA5 19 and HCA90 were highly expressed in 9 9 and 6 9 HCC tissues respectively, PCR showed that they had very low levels of transcript expression in normal liver tissues and other normal tissues, whereas such low-expression transcripts could not be detected in Northern hybridization.HCA5 19 protein was first detected in swollen Tumor patients can induce the body’s immune response, is a new tumor-associated antigen molecules .Among its HCA90 antigen gene for the first time found a new gene whose function may be related to the malignant proliferation of cells, and further study as a Feasibility of Targets for Clinical Cancer Treatment and Diagnosis