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建立了测定痕量阿米卡星制剂的新方法。研究了阿米卡星(AK)与电子受体2,3-二氰-5,6-二氯-1,4-对苯醌(DDQ)之间的荷移反应。在甲醇中,阿米卡星与DDQ在室温(30℃)下可迅速形成荷移络合物,其荧光强度较阿米卡星显著增强,最大激发和发射波长分别红移38 nm和86 nm。阿米卡星含量在0.1~1.8×10-6mol/L范围内与其荧光强度呈良好的线性关系,相关系数r为0.9992,检出限为0.0244 mg/L。本法用于实际样品分析,回收率为96.1%~103%,相对标准偏差(RSD)为1.2%~1.7%。
A new method was established for the determination of trace amikacin preparations. The charge reaction between amikacin (AK) and the electron acceptor 2,3-dicyano-5,6-dichloro-1,4-p-benzoquinone (DDQ) was studied. In methanol, amikacin and DDQ could rapidly form charge-transfer complexes at room temperature (30 ℃), and their fluorescence intensities were significantly enhanced compared with amikacin. The maximum excitation and emission wavelengths were red-shifted by 38 nm and 86 nm, respectively . The linear relationship between the amikacin and its fluorescence intensity was found in the range of 0.1 ~ 1.8 × 10-6mol / L with a correlation coefficient r of 0.9992 and a detection limit of 0.0244 mg / L. The method was applied to the actual sample analysis. The recovery rates ranged from 96.1% to 103% with relative standard deviations (RSDs) of 1.2% -1.7%.