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目的:研究子宫肌瘤模型大鼠造模过程中血浆代谢表型的动态变化及其与组织病理和生化指标的相关性,探讨标志模型成功及药物有效性评价的动态和无损伤性生物标记物指标。方法:于造模过程中4个不同时间点收集大鼠血浆,采用基于核磁共振谱(nuclear magnetic resonance,NMR)的代谢组学方法,测定其NMR,同时进行组织病理学检查和相关生化指标测定。结果:造模不同阶段模型组大鼠血浆的代谢谱与空白对照组比较均有显著差异,随着造模时间的延长,模型组大鼠血浆的代谢谱呈现出不同变化,其变化与子宫组织病理学和免疫组化指标改变相一致。结论:子宫肌瘤模型大鼠血浆代谢谱的谱峰变化能反映造模不同阶段动物的整体病理状态,并且该代谢谱的检测较病理检测便捷、无损伤,可作为子宫肌瘤大鼠模型的早期生物标记物性指标和药物有效性筛选的动态观测指标。
OBJECTIVE: To investigate the dynamic changes of plasma metabolic phenotype during the modeling of uterine fibroids and its relationship with histopathology and biochemical indexes, and to explore the dynamic and non-invasive biomarkers of successful model evaluation and drug efficacy evaluation index. METHODS: Plasma was collected from rats at four different time points during the modeling process. NMR was determined by nuclear magnetic resonance (NMR) based metabonomics. Histopathology and biochemical markers were also determined . Results: Compared with blank control group, the metabolic profile of plasma in model group at different stages of modeling was significantly different. With the extension of modeling time, the metabolic profile of plasma in model group showed different changes, and the changes were similar to those of uterine tissue Pathology and immunohistochemical changes consistent. Conclusion: The peak changes of plasma metabolites in uterine fibroids model can reflect the whole pathological state of animals in different stages of modeling, and the detection of the metabolites spectrum is more convenient than the pathological examination, so it can be used as a model of uterine fibroids in rats Dynamic biomarkers of early biomarkers and drug efficacy screening.