Batocera lineolata Chevrolat,1852 is an important forest pest that is found mainly in China,Vietnam,India,and Japan.The complete mitochondrial genome(mitogenome) sequence of B.lineolata was determined by long polymerase chain reaction(PCR) and conserved primer walking approaches.The results showed that the entire mitogenome is 15418 bp long with 74.48% A+T content.The positions and arrangement of the 37 genes encoded by the genome are identical to the mitogenomes of two other longhorn beetles for which the complete gene content and arrangement are publicly available.All protein-coding genes start with the ATN codon that is a typical initiation codon in insects.All transfer RNAs(tRNAs) were predicted to form the standard clover-leaf structure,except for tRNA Ser(AGN),which lacks the dihydrouridine(DHU) arm.The most unusual feature that was found was the use of TCT as the tRNA Ser(AGN) anticodon instead of the GCT that is used in most other arthropods.The lack of tandem repeat motif in the 735 bp long A+T-rich region was another unusual feature of the B.lineolata mitogenome.The short,highly conserved polythymidine stretch that was previously described in the Orthoptera and Diptera orders was also present in the A+T-rich region of the B.lineolata(order Coleoptera) mitogenome.The sequence and annotation of the mitogenome of B.lineolata will provide further insights into the diversity and evolution of the Cerambycidae family of long-horned beetles.The B.lineolata mitogenome sequence has been deposited in GenBank(http://www.ncbi.nlm.nih.gov/Genbank/) under accession number JN986793. Batocera lineolata Chevrolat, 1852 is an important forest pest that is mainly found in China, Vietnam, India, and Japan. The complete mitochondrial genome (mitogenome) sequence of B. lineolata was determined by long polymerase chain reaction (PCR) and conserved primer walking approaches.The results showed that the entire mitogenome is 15418 bp long with 74.48% A + T content. positions and arrangement of the 37 genes encoded by the genome are identical to the mitogenomes of two other longhorn beetles for which the complete gene content and arrangement are publicly available. All protein-coding genes start with the ATN codon that is a typical initiation codon in insects. All transfer RNAs (tRNAs) were predicted to form the standard clover-leaf structure, except for tRNA Ser (AGN), which lacks the dihydrouridine (DHU) arm.The most unusual feature that was found was the use of TCT as the tRNA Ser (AGN) anticodon instead of the GCT that is used in most other arthropods. The lack of tandem repeat motif in t he 735 bp long A + T-rich region was another unusual feature of the B.lineolata mitogenome.The short, highly conserved polythymidine stretch that was previously described in the Orthoptera and Diptera orders was also present in the A + T-rich region of the B. lineolata (order Coleoptera) mitogenome. The sequence and annotation of the mitogenome of B. lineolata will provide further insights into the diversity and evolution of the Cerambycidae family of long-horned beetles. B.lineolata mitogenome sequence has been deposited in GenBank (http://www.ncbi.nlm.nih.gov/Genbank/) under accession number JN986793.