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采用DTNB显色法 ,研究平原和模拟 40 0 0m高原缺氧条件下梭曼对犬、大鼠组织及全血AChE活力的影响。结果显示 :(1)缺氧可抑制AChE活性。(2 )模拟高原条件下梭曼对两种动物AChE的抑制和平原条件下一样 ,均为持续性抑制 ,中毒后 2hAChE仍无明显回升。 (3)缺氧复合梭曼中毒对犬全血AChE的抑制速度和抑制程度都较平原中毒组有增高的趋势。而对于大鼠组织来说 ,其AChE的抑制速度明显快于平原中毒组 ,抑制程度也偏重 ,且存在一定的剂量效应关系。(4)缺氧复合梭曼中毒对大鼠全血AChE的抑制峰提前出现 ,但抑制程度相对轻些。以上结果表明在缺氧条件下血液中AChE的活性变化与梭曼的毒性并不呈平行关系 ,而脑组织中AChE的变化一定程度上可反映梭曼的毒性程度。
The DTNB method was used to study the effects of soman on plateau and simulated AChE activity in dogs and rats and in whole blood under plateau anoxia plateau of 400 m altitude. The results showed that: (1) hypoxia can inhibit AChE activity. (2) Under the condition of simulated plateau, the inhibitory effect of soman on AChE in both animals was the same as that in the plain condition, and no significant increase was observed at 2h after poisoning. (3) The inhibitory rate and inhibition of AChE by anaerobic combined soman intoxication in canine whole blood were higher than those in placental poisoning group. For rat tissues, the inhibition rate of AChE was significantly faster than that of placental poisoning group, and the degree of inhibition was also heavier, and there was a certain dose-response relationship. (4) The inhibition peak of AChE in rat whole blood appeared by anoxia combined with soman poisoning, but the degree of inhibition was relatively low. The above results show that the change of AChE activity in blood is not parallel to that of soman under hypoxic conditions, but the change of AChE in brain tissue can reflect the degree of soman’s toxicity to some extent.