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对灯笼椒、牛角椒、羊角椒、线形椒和朝天椒等辣(甜)椒游离小孢子的培养与观察表明:在雄核发育过程中,分离后仍膨大的小孢子能够继续发育,皱缩的小孢子则死亡;小孢子最初的细胞分裂有均等和不均等两种方式;脱分化的小孢子可经历胚胎发生和器官发生两种发育途径,二者之间可相互转换;胚胎发生过程与合子胚发育过程极相似,直到心形胚以前均可观察到胚柄的存在;成熟的胚状体能够萌发,但胚根端生长的能力远远比胚芽端强;观察到胚状体发生不同步及多种畸形发育形式;小孢子在分裂早期如果突破花粉壁会形成薄壁细胞团,继续发育为愈伤组织,进而转向器官发生途径;小孢子愈伤组织易形成不定根,可形成次生胚状体,未观察到不定芽的发生。不同类型辣(甜)椒游离小孢子的雄核发育与胚胎发生程序相同。
The microspore culture and observation of pepper (Capsicum annuum L.), croaker (Capsicum annuum L.), Chinese croaker (Capsicum annuum L.), Zingiber officinale and Chaotianjiao pepper showed that during microspore development, Of the microspores die; microspore initial cell division is equal and unequal in two ways; dedifferentiated microspores can undergo embryonic and organogenesis of two developmental pathways, the two can be converted between the two; embryogenesis and The process of zygotic embryo development is very similar, until the embryo can be observed before the heart-shaped embryos; mature embryoid body can germinate, but the ability of the radicle growth is far stronger than the embryo; embryoid body was observed not to occur Synchronization and a variety of forms of deformity development; microspores in the early division of the wall if the break through the pollen will form parenchyma cell clusters continue to develop into callus, and then turned to organ pathways; microspores callus easy to form adventitious roots, the formation of secondary Embryoid body, no adventitious buds were observed. Different types of spicy (sweet) pepper free microspores of the same development and embryogenesis.